pollen be grown on an agar medium to produce haploid plants!!?
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josh.fittler asked:
pollen be grown on an agar medium to produce haploid plants!! how can this be done differently to normal tissue culture( i do normal tissue culture) ie as in different hormones and methods?
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pollen be grown on an agar medium to produce haploid plants!! how can this be done differently to normal tissue culture( i do normal tissue culture) ie as in different hormones and methods?
Question posted courtesy of: Caffeinated Content - Members-Only Content for WordPress
November 17th, 2007 at 4:34 am
This is called “pollen culture”, and it is used to produce haploid plantlets. If you search Google for “pollen culture”, you will find a lot of information. The medium is agar, and you will need plant hormones. Here is one recipe, there are more in the sources links:
Chu’s N6 medium (available from Sigma-Aldrich) supplemented with NAA, a synthetic auxin (2-4 mg/1), kinetin (0.3 mg/1), casein hydrolysate (1 g/1), and 5% sucrose at pH 5.7