how to stain pollen grains for light microscopy / fluorescence microscopy/ confocal microscopy ?

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pollen
nanobot asked:

i am in need of proper protocols for staining pollen grains (pollen exines) using flourescent and non-fluorescent stains.
I SM IN NEEEEEEEEEEEEEEED OF AN ANSWER.

ANYONE THERE WHO CAN HELP ME!
I AM IN NEED OF TA METHOF TO STAIN POLLEN EXINE

ANY EXPERT OUT THERE


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    • This entry was posted on Wednesday, October 3rd, 2007 at 10:26 pm and is filed under Health. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.

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    2 Responses to “how to stain pollen grains for light microscopy / fluorescence microscopy/ confocal microscopy ?”

    1. smalleyessharpviews Says:
      October 5th, 2007 at 4:50 pm

      no need of staining.

    2. plantmd Says:
      October 8th, 2007 at 7:51 pm

      You can use lactophenol blue.. but other than that, especially for florescence, I am not sure. Lactohpenol blue is used typically in staining fungal structures and is a very common staining chemical.

      There are others like TritonX that are used to stain viruses, but this is a more complex method of staining that requires a few chemicals and heating the sample.

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